Soil Foodweb Inc.     Compost Tea Analysis     Organism Ratios                                                                                                                                                                                                               
    1128 NE 2nd St.    Suite 120     Client:    Alaska Humus       Sample Treatment Total Fungal Active to Active to Active Fungal Plant Available Root-Feeding      
    Corvallis, OR   97330       Jeff Lowenfels,       #   to Total Fungal Total Bacterial to Active N Supply Nematode      
    Phone:   541-752-5066       711 M St             Total Bacterial Biomass Biomass Bacterial from Predators Presence      
    FAX 541-752-5142       Anchorage, Alaska     99501                                                                                 Biomass                                                                                            Biomass                  (lbs/ac)                                                     
    E-Mail:   info@soilfoodweb.com                95550 Fungal Humus HS 0.011 0.99 0.029 0.37 NR NR      
                jeff@gardener.com                            
              Sample Received:1/28/03 Date Mailed:    Jan 30, 2003     Great Nearly all the fungi OK OK          
              Tea   recipe:   See below           bacteria, are actively              
              Invoice #:               adequate fungi growing              
Organism Biomass Data                                                                                  Grower:                                
Sample Treatment Tea Active Total   Active Total                 Total         Total                           
#   Volume Bacterial Bacterial Fungal Fungal Hyphal     Protozoa       Nematode                      
    (mL) Biomass Biomass Biomass Biomass Diameter                             Numbers /mL                        Numbers                                                                                                                                                                                                                                                                                  
                                                                                              (µg/mL)                                     (µg/mL)                                    (µg/mL)                                (µg/mL)                    (µm)       Flagellates    Amoebae         Ciliates          (#/mL)                          
95550 Fungal Humus HS 1.00 92.2 3232 34.4 34.6 2.5 NR NR NR NR                        
                          Desired   (1) (2) (2) (3) (4) (5)      
      In Excellent Excellent Excellent OK   WOW!   Great fungi!                Range                                                                                                                                                                                                                                    
      good                   (1)   For example, for soil drenches, with the following plants, Grass:0.5-1.5; Berries, Shrubs, grape: 2-5; Deciduous Trees: 5-10; Conifer: 10-100.      
      range                         For foliar sprays, ratio should be 0.01 to 0.05 because foliar sprays are typically best strongly bacterial-dominated.        
                          (2)   Teas in general have high ratios of active to total fungi, since what fungi are present are actively growing, but with low total biomass.        
                                 In general, fungi don't like to grow suspended in liquid unless a solid surface is present.   Bacterial activity must be high, above 25%.      
Bold                         (3)   Teas generally have lower fungal biomass than bacteria, so this value is typically less than 10%.   It is desirable to make this ratio as high as      
means                                 possible.                  
low                                                                                 (4)   Based on release of N from protozoan and nematode consumption of bacteria and fungi.   Often protozoa and nematodes compete for food resources.    
  Desired 1 10 - 150 - 2 - 2 -   1,000 1,000 20-              2 -           When one is high, the other may be low.   Also, if predator numbers are high, the prey may have low numbers        
                               Range                                                      150                          300                     10                    20                (A)                                                                     50                        10      (5)   Identification to genus.   For species identification of root-feeders, send samples to local parasitic nematology lab.        
(A)   Hyphal diameter of 2.0 indicates mostly actinomycete hyphae, 2.5 indicates community is mainly ascomycete, typical soil fungi for grasslands,                      
         diameters of 3.0 or higher indicate community is dominated by highly beneficial fungi, a Basidiomycete community.       Fungal foods are humic acids, woody mulch, fungal compost, fungal compost tea.              
Temperature of brewing, type of water (chlorine will kill organisms), type of compost and type of brewer used must be considered in determining                        
the set of organisms in the tea.   See the Compost Tea Manual for complete information.   Tea assessment should be accompanied by leaf                        
organism assessment to see if there were effects of spraying or diluting in the sprayer.   Pesticide use, fertilizer use, tillage, irrigation, etc., affect soil                      
and foliar effectiveness.   One report is sent to the mailing address on the submission form.                                
                                               
Recipe: 1 T Humisolve, 3 T sol. kelp; 13 hrs.   Hi temp 82F.    Tech Notes: Most total fungi is new growth active fungi. Lots of spores with small spurt of growth. NJO.